NIKON A1R双光子荧光显微镜

生产公司：NIKON（日本）

仪器型号：A1R MP

主要性能指标：

1、可见光激光器部分：

Ar离子激光器: 波长488nm;

绿光源激光器: 波长543nm；

红光源激光器：波 长640nm

2、红外双光子飞秒激光器，波长 690-1040nm,

3、扫描系统：线扫描速度15000线/秒；扫描速度：512 X 512条件下30幅/秒，共聚焦点扫描分辨率: 4096 X 4096像数点。

4、物镜：全部配置APO共聚焦显微镜专用高分辨率、高透过率物镜, 配置全套 DIC棱镜

16倍APO共聚焦专用物镜，数值孔径0.80

25倍 APO 共聚焦专用水镜，数值孔径1.10,

工作距离 2.0 mm

40倍 APO 共聚焦专用水镜,数值孔径 0.8

60倍 APO 共聚焦专用水镜,数值孔径 1.1

适用范围：

1、细胞间通讯的研究；

2、细胞物理化学测定；

3、多点瞬间光活化光刺激功能；

4、电生理研究；

      5、pH、细胞内离子分析；

      6、在体活体观察等；

仪器功能特色：

1、光刺激和图像扫描能同步触发、同步记录，并能进行电生理信号和共聚焦图像信号的叠加。提供光刺激和扫描事件的同步TTL输出；

2、扫描速度：512 X 512条件下30幅/秒；

      3、利用共振扫描器，实现双光子刺激（光遗传激活,FRAP,FLIP，uncage等）的同时进行单光子video成像。

      4、具有红外飞秒激光自动准直功能。

放置地点：上海市华山路1954号yd12300云顶线路教三楼Med-X研究院117室

联系人：顾翔

电话：13764512572 

Email：Hallelujah@sjtu.edu.cn

Nikon A1R Multiphoton Fluorescence Confocal Microscope

Manufacturing Company: NIKON (Japan)

Instrument Model: A1R MP

Main performance index:

1. The part of visible light laser:

2. Ar ionic laser: wavelength 488nm;

3. Green light source laser: wavelength 543nm;

4. Red source laser: wavelength 640nm

5. Infrared two-photon femtosecond laser: wavelength 690-1040nm,

6. Scanning system: the speed of line scanning is 15000 lines/second;

7. Scanning speed: 30 images/second under the condition of 512 X 512 pixels，confocal scanning resolution: 4096 X 4096 pixels.

8. Objective lenses: All APO confocal microscope is equipped with special high resolution and high transmittance objectives lenses, and a full set of DIC prisms.

16X APO confocal objective lens, numerical aperture 0.80

25X APO confocal objective lens, numerical aperture 1.10,

Working distance 2.0mm

40X APO confocal special water objective lens, numerical aperture 0.8

60X APO confocal special water objective lens, numerical aperture 1.1

Sphere of application:

1. The study of intercellular communication;

2. Physical and chemical determination of cells;

3. The function of multi-point instantaneous photo-activation and photo-stimulation;

4. Electrophysiological research;

5. pH and intercellular ionic analysis;

6. In vivo and viviperception observation, etc.

Instrument features:

1. Light stimulation and image scanning can trigger and record synchronously, and signals of electrophysiological and confocal image can be superimposed. This instrument also can provide synchronous TTL output of light stimulation and scan events;

2. Scanning speed: 30 images/second under the condition of 512 X 512 pixels;

3. Multiphoton stimulation and the image formation of single photon video can be realized simultaneously (optogenetic activation, FRAP, FLIP, UNCAGE, etc.) using resonance scanner.

4. Possessing the automatic calibration function of infrared femtosecond laser.

Location: Room 117, MED-X Research Institute, No.3 Teaching Building, Shanghai Jiaotong University, No. 1954 Huashan Road, Shanghai

Contact: Shawn Gu

Contact Information:

Telephone: 13764512572

Email: Hallelujah@sjtu.edu.cn